|
|
Registro completo
|
Biblioteca (s) : |
INIA Tacuarembó. |
Fecha : |
07/07/2020 |
Actualizado : |
09/07/2020 |
Tipo de producción científica : |
Documentos |
Autor : |
DÍA DE CAMPO: ARROZ - DEAMBROSI, E.; BLANCO, P.; PÉREZ DE VIDA, F; ZORRILLA DE SAN MARTÍN, G.; ACEVEDO, A.; AVILA, S.; MÉNDEZ, R.; BLANCO, F.; ROEL, A.; PÍRIZ, M. |
Título : |
Trabajos presentados |
Fecha de publicación : |
1993 |
Fuente / Imprenta : |
Treinta y Tres (Uruguay): INIA, 1993. |
Páginas : |
51 p. |
Idioma : |
Español |
Notas : |
INIA Treinta y Tres.Estación Experimental del Este |
Thesagro : |
APLICACION DE ABONOS; ARROZ; CLIMATOLOGIA; ENFERMEDADES DE LAS PLANTAS; ENSAYOS DE VARIEDADES; ESCARDA; FITOMEJORAMIENTO; FITOPATOLOGIA; MALEZAS; MANEJO DEL CULTIVO; RIEGO. |
Asunto categoría : |
-- |
Marc : |
LEADER 00982nam a2200361 a 4500 001 1061205 005 2020-07-09 008 1993 bl uuuu u0uu1 u #d 100 1 $aDÍA DE CAMPO: ARROZ - DEAMBROSI, E. 245 $aTrabajos presentados$h[electronic resource] 260 $aTreinta y Tres (Uruguay): INIA$c1993 300 $a51 p. 500 $aINIA Treinta y Tres.Estación Experimental del Este 650 $aAPLICACION DE ABONOS 650 $aARROZ 650 $aCLIMATOLOGIA 650 $aENFERMEDADES DE LAS PLANTAS 650 $aENSAYOS DE VARIEDADES 650 $aESCARDA 650 $aFITOMEJORAMIENTO 650 $aFITOPATOLOGIA 650 $aMALEZAS 650 $aMANEJO DEL CULTIVO 650 $aRIEGO 700 1 $aBLANCO, P. 700 1 $aPÉREZ DE VIDA, F 700 1 $aZORRILLA DE SAN MARTÍN, G. 700 1 $aACEVEDO, A. 700 1 $aAVILA, S. 700 1 $aMÉNDEZ, R. 700 1 $aBLANCO, F. 700 1 $aROEL, A. 700 1 $aPÍRIZ, M.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Tacuarembó (TBO) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
23/09/2019 |
Actualizado : |
23/09/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
B - 1 |
Autor : |
COUTO, D.; STRANSFELD, L.; ARRUABARRENA, A.; ZIPFEL, C.; LOZANO-DURÁN, R. |
Afiliación : |
DANIEL COUTO, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; LENA STRANSFELD, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CYRIL ZIPFEL, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; ROSA LOZANO-DURÁN, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; Shanghai Institutes of Biological Sciences, Shanghai Center for Plant Stress Biology (PSC), Chinese Academy of Sciences, China. |
Título : |
Broad application of a simple and affordable protocol for isolating plant RNA. (Short Report). |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
BMC Research Notes, 16 April 2015, Volume 8, Issue 1, Article number 154. OPEN ACCESS. |
ISSN : |
1756-0500 |
DOI : |
10.1186/s13104-015-1119-7 |
Idioma : |
Inglés |
Notas : |
Article history: Published on: 16 April 2015. |
Contenido : |
Abstract View references (4)
Background: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. Findings: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. Conclusions: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide.
© 2015 Couto et al.; licensee BioMed Central. |
Palabras claves : |
Affordable; Arabidopsis; Protocol; RNA; Tomato; Wheat. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13321/1/Couto-et-al.-2015.-BMC-Research-Notes.pdf
https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-015-1119-7
|
Marc : |
LEADER 02324naa a2200277 a 4500 001 1060208 005 2019-09-23 008 2015 bl uuuu u00u1 u #d 022 $a1756-0500 024 7 $a10.1186/s13104-015-1119-7$2DOI 100 1 $aCOUTO, D. 245 $aBroad application of a simple and affordable protocol for isolating plant RNA. (Short Report).$h[electronic resource] 260 $c2015 500 $aArticle history: Published on: 16 April 2015. 520 $aAbstract View references (4) Background: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. Findings: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. Conclusions: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide. © 2015 Couto et al.; licensee BioMed Central. 653 $aAffordable 653 $aArabidopsis 653 $aProtocol 653 $aRNA 653 $aTomato 653 $aWheat 700 1 $aSTRANSFELD, L. 700 1 $aARRUABARRENA, A. 700 1 $aZIPFEL, C. 700 1 $aLOZANO-DURÁN, R. 773 $tBMC Research Notes, 16 April 2015, Volume 8, Issue 1, Article number 154. OPEN ACCESS.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|